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3 plasmids (pGL1124, pGL1224 and pGL1217) ended up made to enable the substitution of one allele of CYC9Lister 427 cells. Trypanosomes had been transfected with Just about every plasmid individually, after which you can subjected into a second transfection with one of many other plasmids, with a distinct resistance gene, with all pairwise combos pe
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At the end of the experiments, the organ was fixed with 4% paraformaldehyde overnight and embedded in paraffin. Paraffin sections have been Slice into the slice that has a thickness of 4 ?m. H&E staining was accustomed to evaluate inflammatory infiltration. The quantity of CD45 was analyzed by immunohistochemistry.Being familiar with how physiolog
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